Alaabta

Faahfaahinta Alaabta:

Innovita® 2019-nCoV IgM/IgG Tijaabada waxaa loogu talagalay ogaanshaha iyo la socodka cudurada faafa ee cudurada uu keeno novel coronavirus (2019-nCoV).ORF1ab iyo N hiddo-wadaha 2019-nCoV si tayo leh ayaa looga helaa suufka cunaha iyo muunadaha dareeraha dheecaanka alveolar ee laga soo aruuriyay kiisaska looga shakisan yahay oof wareenka, bukaanada qaba kiisaska la tuhunsan yahay, kuwa kale ee u baahan in la ogaado.
Natiijooyinka baaritaanka xirmadan waxaa loogu talagalay tixraac bukaan oo keliya.Waxaa lagu talinayaa in la sameeyo falanqayn dhamaystiran oo ku saabsan xaaladda ku salaysan calaamadaha bukaan-socodka iyo shaybaarka kale ee shaybaarka.

Mabda'a:

Qalabkani waxa uu isticmaalaa hal-tallaabo rogaal celinta silsiladda polymerase (RT-PCR) Tignoolajiyada ogaanshaha si loo beegsado novel coronavirus (2019-nCoV) ORF1ab hiddo-wadaha, hidda-wadaha N, iyo taxanaha tixraaca gudaha ee bini'aadamka.Tilmaamayaal gaar ah iyo baarayaal taqman ayaa lagu nashqadeeyay gobollada la xafiday.

Halabuurka:

Fiiro gaar ah: 1. Qaybo kala duwan oo reagents ah waa in aan la isku qasin.
2. Koontarool wanaagsan iyo kontaroolo togan looma baahna in la soo saaro

Habka Imtixaanka:

1. Soo saarista aashitada nukliyeerka:
Xirmooyinka soo saarista RNA ee ganacsiga ayaa diyaar ah, soo saarista birta birta ah iyo soo saarista tiirarka lafdhabarta ayaa lagula talinayaa xirmadan.
2. Diyaarinta isku darka falcelinta:

● Ka saar falcelinta 2019-nCoV Mix A/B oo ku hay heerkulka qolka ilaa inta aanad barafayn;
● Qaado qaybaha u dhigma (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) oo isku qas, ka dibna ku dar falcelinta PCR kasta oo leh 20μL/tubo;
Ku dar 5μL ee template RNA ama kontoroolka xun ama xakamayn togan, ka dibna dabool daboolka tuubada;
● Geli tuubada falcelinta gudaha qalabka PCR ee fluorescence, oo samee xakamaynta taban/togan iyo cabbirada muunada falcelinta RT-PCR sida waafaqsan hab-raaca qalabka.
● Diiwaangeli qaabka meelaynta

3.RT-PCR borotokool:

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