Alaabta

Faahfaahinta Alaabta:

Innovita® 2019-nCoV IgM/IgG Tijaabada waxaa loogu talagalay in lagu ogaado cabbirka ka-hortagga ka-hortagga ka-hortagga novel coronavirus (2019-nCoV) ee serum bini'aadamka, balaasmaha ama dhiigga oo dhan (dhiigga cidhifka ama xididdada dhiigga oo dhan) shaybaarrada.
2019-nCoV waxaa ku jira afar borotiinno dhisme oo waaweyn: borotiinka S, borotiinka E, borotiinka M iyo borotiinka N.Gobolka RBD ee borotiinka S wuxuu ku xidhmi karaa soo-dhoweeyaha unugga aadanaha ee ACE2.Daraasaduhu waxay muujiyeen in shaybaarada dadka ka soo kabsaday infekshanka coronavirus-ka cusub ay u fiican yihiin inay ka takhalusaan antibody.Ogaanshaha ka hortagga antibody-ka ayaa loo isticmaali karaa si loo qiimeeyo saadaasha caabuqa fayraska iyo qiimaynta saamaynta tallaalka ka dib.

Mabda'a:

Xirmada ayaa ah qiyaasta chromatography ee quantum dot immunofluorescence chromatography si loo ogaado 2019-nCoV RBD gaar ah IgG ka-hortagga unugyada difaaca jirka ee serum, balaasmaha ama dhiigga oo dhan (dhiigga caarada iyo dhiigga oo dhan) shaybaarrada.Ka dib muunada si fiican loo mariyo muunada, haddii xoogga saarista unugyada difaaca jirka ay ka sarreyso xadka ugu hooseeya ee ogaanshaha, unugyada difaaca jirka ee RBD ee gaarka ah ayaa ka falcelin doona qayb ama dhammaan RBD antigen ee lagu calaamadiyay microspheres dhibicda quantum si ay u sameeyaan xarunta difaaca.Markaas xerada difaaca ayaa u guuri doonta xuubka nitrocellulose.Marka ay gaadhaan aagga tijaabada (T line), xaruntu waxay la falgeli doontaa jiirka ka-hortagga bini-aadmiga IgG (γ silsiladda) ee ku dahaaran xuubka nitrocellulose oo waxay sameeyaan xariiq fluorescent ah.Ku akhri qiimaha calaamadaha fluorescence adigoo isticmaalaya falanqeeyaha immunoassay ee fluorescence.Qiimaha calaamaduhu wuxuu u dhigmaa waxa ku jira ka-hortagga unugyada unugyada ee muunada.
Haddii muunada ay ku jirto RBD gaar ah oo ka hortagta unugyada difaaca jirka iyo haddii kale, khadka kantaroolku waa inuu had iyo jeer ka soo baxaa daaqadda natiijada haddii nidaamka tijaabada si sax ah loo sameeyo oo reagent uu u shaqeeyo sidii loogu talagalay.Marka digaagga IgY antibody ee ku calaamadsan quantum dot microspheres u haajiraan xariiqda kantaroolka (C line), waxaa qaban doona riyaha ka hortagga digaagga IgY ee lagu sii dahaaray khadka C, waxaana la sameeyay xariiq fluorescent ah.Xariiqda xakamaynta (khadka C) waxa loo istcimaalayaa hab-raac habraaceed.

Halabuurka:

Habka Imtixaanka:

● Ururinta Dhiiga cirifka faraha

● Ku akhri natiijada adigoo isticmaalaya falanqeeyaha fluorescence